STEPS OF DNA REPLICATION

( INITIATION )



1) Breaking of hydrogen bond between bases of double helix strand of DNA. HELICASE is the enzyme responsible to unwind the double helix strand. Intiation point where splitting starts is called origin of replication. The structure that is created is called the Replication Fork.

2) SSB protein also called single stranded binding protein will stabilize and prevent the single stranded dna from recoiling in helix until replicated. Topoisomerase or Gyrase function is to prevent supercoiling and knot formation during replication.